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ZYMO RESEARCH D5003 EZ-96 DNA Methylation Kit (Shallow-Well) 2 x 96 Rxns
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ZYMO RESEARCH D5003 EZ-96 DNA Methylation Kit (Shallow-Well) 2 x 96 Rxns

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LB.MB.ZYMD5003
ZYMO RESEARCH D5003 EZ-96 DNA Methylation Kit (Shallow-Well) 2 x 96 Rxns
Streamlined, proven procedure for bisulfite conversion of DNA.
Desulphonation and recovery of bisulfite-treated DNA with a 96-well spin-column plate.
Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
ZYMO RESEARCH D5003 EZ-96 DNA Methylation Kit (Shallow-Well) 2 x 96 Rxns 
The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA. Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulphonation technology eliminates otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # D5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion >99%
Elution Volume ≥ 15 µl for Deep-well
≥ 30 µl for Shallow-well
Equipment Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input 500 pg - 2 µg of DNA
Processing Time 12-16 hours
Recovery >80%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
Supplemental Info

ZYMO RESEARCH D5003 EZ-96 DNA Methylation Kit (Shallow-Well) 2 x 96 Rxns
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ZYMO RESEARCH D5003 EZ-96 DNA Methylation Kit (Shallow-Well) 2 x 96 Rxns ZYMO RESEARCH D5003 EZ-96 DNA Methylation Kit (Shallow-Well) 2 x 96 Rxns, ZYMO RESEARCH D5003 EZ-96 DNA Methylation Kit (Shallow-Well) LB.MB.ZYMD5003
ZYMO RESEARCH D5003 EZ-96 DNA Methylation Kit (Shallow-Well) 2 x 96 Rxns

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