The SensiFAST™ SYBR & Fluorescein Kit has been developed for fast, highly accurate real-time PCR and has been validated on real-time PCR platforms that require the passive reference dye fluorescein for collection of dynamic well factors.

A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR & Fluorescein Kit produces reliable assay results under fast thermal cycling conditions. An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.

The SensiFAST SYBR & Fluorescein Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Applications

Gene expression analysis
DNA / RNA target detection
miRNA profiling / quantification
Copy number variation (CNV) analysis

Product Highlights

Reproducible – consistent results between technical replicates for increased confidence in results
Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
Sensitive – reliable quantification of low abundance targets and scarce samples
Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types
Fast – delivers reproducible, accurate assay results in as little as 30 minutes

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Bioline BIO-96005 SensiFAST SYBR & Fluorescein Kit 500 x 20 µl Reactions

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