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Merck 109215 Ziehl-Neelsen carbol-fuchsin solution for microscopy Ziehl-Neelsen Karbol-Fuksil Çözeltisi 500 ml
Category
Brand
Stock Code
LB.M.109215.0500
Kısa Bilgi
Synonym(s): Carbol fuchsin solution NACRES: NA.74 UNSPSC Code: 41116130
| Ziehl-Neelsen boyama |
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Merck 109215 Ziehl-Neelsen carbol-fuchsin solution for microscopy Ziehl-Neelsen Karbol-Fuksil Çözeltisi 500 ml
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| Asit-fact bakteri için sıcak boyama, metilen mavisi ile counter boyama |
| Merck 109215 Ziehl-Neelsen Karbol-Fuksil Çözeltisi 500 mL |
| Synonym(s): Carbol fuchsin solution NACRES: NA.74 UNSPSC Code: 41116130 |
|
PROPERTIES
form liquid
IVD for in vitro diagnostic use technique(s) microbe id | staining: suitable transition temp flash point 47 °C density 0.99 g/cm3 at 20 °C application(s) clinical testing, diagnostic assay manufacturing, hematology, histology storage temp. 15-25°C Quality Level 400 DESCRIPTION Description Ziehl-Neelsen carbolfuchsin solution - for microscopy, is a ready-to use solution used in the Ziehl-Neelsen hot staining for the microscopic investigation of acid-fast bacteria(AFB). The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Product number 1.00327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use Löfföer′s methylene blue solution(Cat. no. 101287) is used for counterstaining. Those three solutions are each 500 ml and are contained in the AFB-color modified staining kit (Product number 1.00497). The 500 ml bottle of Ziehl-Neelsen carbolfuchsin solution provides approx. 250 stainings. Pretreatment of the specimens with Sputofluol™ dissolves the bacteria from the surrounding viscid sputum and cell material. Ziehl-Neelsen carbolfuchsin solution - for microscopy is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage. Application For Microscopy. Ready-to-use staining solution for the purpose of bacteriological and histological investigation of sample material of human origin. Legal Information SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany |