Merck 109218 Gram's crystal violet solution 25 L

Category

Bakteriyoloji Boya Kitleri

Brand

MERCK

Stock Code

LB.M.109218.9025

Sepete Ekle

Teklif Sepetine Ekle

Gram Boyası

Gram boyama bakterilerin gram pozitif ve gram negatif tiplere ayrıştırılmasını sağlar. Bu proseste, anilin boyası hücre duvarında bir iyot-boya kompleksi oluşturmak üzere iyot ile reaksiyona girer. Gram pozitif bakteriler söz konusu olduğunda, iyot-boya kompleksi, alkol ya da aseton gibi boya giderici maddeler kullanılarak hücreden atılamaz; bu nedenle, hücre mavi-viyole rengini korur. Gram-negatif bakteriler söz konusu olduğunda, iyot-boya kompleksi çözünür ve hücre safranin oranj ya da karbol fusin ile pembe ila kırmızı arası bir renk elde etmek üzere karşıt boyamaya tabi tutulabilir.

Merck 109218 Gram's crystal violet solution
Catalogue Number	109218
Description	Gram's crystal violet solution
Overview	Gram's crystal violet solution for the Gram staining method, is a ready-to-use solution used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with Gram's crystal violet solution (Cat. No.109218), an aniline dye. After the treatment with iodine solution (Lugol’s solution stabilized with PVP, Cat No.100567), a dye-iodine complex will form. During the decolorizing step (Gram’s decolorizing solution, Cat. No. 110218), this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with safranine solution (Gram's safranine solution (Cat.no. 109217) and will then appear pink to red. A bottle with 500 ml Gram's crystal violet solution is sufficient for staining up to 250 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Product Information
HS Code	3212 90 00
Quality Level	MQ400

Applications
Application	for the Gram staining method

Physicochemical Information
Density	0.99 g/cm3 (20 °C)
Flash point	47 °C

Safety Information according to GHS
Hazard Pictogram(s)
Hazard Statement(s)	H226: Flammable liquid and vapour. H412: Harmful to aquatic life with long lasting effects.
Precautionary Statement(s)	P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P233: Keep container tightly closed. P240: Ground and bond container and receiving equipment. P241: Use explosion-proof electrical/ ventilating/ lighting equipment. P242: Use non-sparking tools. P273: Avoid release to the environment.
Signal Word	Warning
Storage class	3 Flammable liquids
WGK	WGK 2 obviously hazardous to water
Disposal	28 Aqueous solutions: Container D.

Safety Information
Categories of danger	flammable, dangerous for the environment

Storage and Shipping Information
Storage	Store at +15°C to +25°C.

Transport Information
Declaration (railroad and road) ADR, RID	Kein Gefahrgut
Declaration (transport by air) IATA-DGR	No Dangerous Good
Declaration (transport by sea) IMDG-Code	No Dangerous Good

Specifications
Suitability for microscopy	passes test
gram-positve microorganisms	blue-violet
gram-negative microorganisms	pink to red

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