MERCK 110426 Coliform Agar acc. to ISO 9308-1 Chromocult® 500 gr
Catalogue Number	110426
Overview	Chromocult® Coliform Agar facilitates and speeds up your processes. It allows the simultaneous detection, enumeration and confirmation of coliforms and E. coli in water within 24 hours. Chromocult® Coliform Agar comes in a granulated format and is the only chromogenic culture medium which was used for validation to prepare the new ISO 9308-1 (2014) version.

Product Information
HS Code	3821 00 00

Physicochemical Information
pH value	6.6 - 7.0 (26.5 g/l, H₂O, 25 °C)
Solubility	26.5 g/l

Safety Information according to GHS
Hazard Pictogram(s)
Hazard Statement(s)	H315: Causes skin irritation. H318: Causes serious eye damage.
Precautionary Statement(s)	P280: Wear eye protection. P302 + P352: IF ON SKIN: Wash with plenty of soap and water. P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P313: Get medical advice/ attention.
Signal Word	Danger
Storage class	10 - 13 Other liquids and solids
WGK	WGK 2 obviously hazardous to water
Disposal	3 Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Storage and Shipping Information
Storage	Store at +15 °C to +25 °C.

Specifications
Appearance (clearness)	slightly turbid to turbid
Appearance (colour)	yellowish to yellowish-brown
pH-value (25 °C)	6.6 - 7.0
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))
Inoculum on reference medium (Enterobacter aerogenes ATCC 13048)
Inoculum on reference medium (Citrobacter freundii ATCC 43864 (WDCM 00006))
Colony count (Escherichia coli ATCC 25922 (WDCM 00013))
Colony count (Escherichia coli ATCC 8739 (WDCM 00012))
Colony count (Enterobacter aerogenes ATCC 13048)
Colony count (Citrobacter freundii ATCC 43864 (WDCM 00006))
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013))	≥ 70 %
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))	≥ 70 %
Recovery on test medium (Enterobacter aerogenes ATCC 13048)	≥ 70 %
Recovery on test medium (Citrobacter freundii ATCC 43864 (WDCM 00006))	≥ 70 %
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009))	partial inhibition
Growth (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024))	no limit
Colonies (Escherichia coli ATCC 25922 (WDCM 00013))	dark-blue to violet
Colonies (Escherichia coli ATCC 8739 (WDCM 00012))	dark-blue to violet
Colonies (Enterobacter aerogenes ATCC 13048 (WDCM 00175))	pink to red
Colonies (Citrobacter freundii ATCC 43864 (WDCM 00006))	pink to red
Colonies (Pseudomonas aeruginosa ATCC 10145 (WDCM 00024))	colourless
Incubation: 21 ± 3 hours at 36 ± 2 °C A recovery rate of 70 % is equivalent to a productivity value of 0.7. The indicated colony counts result from the sum of a triple determination. Reference media: Tryptic Soy Agar

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Merck 110426 Coliform Agar Acc. To Iso 9308-1 Chromocult® 500 gr

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