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Sigma-Aldrich 1.07393 Proteinase K (from Tritirachium album) solution in Tris/HCl pH 7.5; 0.01 mol/l; 600 mAnson-U/ml; for molecular biology EC 3.4.21.14 10 mL
Brand
Stock Code
LB.SA.1073930010
Kısa Bilgi
Synonym(s): ProK, native proteinase K solution, non-specific protease NACRES: NA.51 UNSPSC Code: 12352204
| Sigma-Aldrich 1.07393 Proteinase K |
| Synonym(s): ProK, native proteinase K solution, non-specific protease NACRES: NA.51 UNSPSC Code: 12352204 |
|
PROPERTIES
Product Name Proteinase K, (from Tritirachium album) solution in Tris/HCl pH 7.5; 0.01 mol/l; 600 mAnson-U/ml; for molecular biology EC 3.4.21.14
form liquid pH 7.5 (25 °C in H2O, undiluted) density 1.10 g/cm3 at 20 °C storage temp. 2-8°C Quality Level 400 DESCRIPTION Analysis Note Appearance (colour): colourless Appearance (clearness): clear Activity (hemoglobin; pH 7.5; 37 °C): ≥ 600 mAnsonU/ml Spec. activity (calc. on protein): ≥ 40.0 mAnsonU/mg DNases (Nicking activity; pBR 322; 6 h; 37 °C): not detectable RNases (RNA; 2 h; 37°C): not detectable Colony count (aerobic bacteria): ≤ 10 CFU/ml Application Proteinase K has been used: - to de-crosslink immunoprecipitated samples - to treat the poly-L-lysine coated slides of colon tissues for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay - - in in situ hybridization Biochem/physiol Actions Proteinase K catalyzes the hydrolysis of esters and peptide bonds. It is used with non-aqueous hydrated solvents for synthesizing peptides. Proteinase K is used to break the cross-linking that develops secondary to formalin fixation and expose the target sequence for primers and polymerase. General description Proteinase K is an endopeptidase that belongs to the subtilisin family of proteinases. The polypeptide chain contains 278 amino acids with the catalytic triad Asp39, His69, Ser224. |