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Sigma-Aldrich B8781 Restriction Enzyme Buffer SB 10 × 5X1 VIAL

Stock Code
LB.SA.B8781-5X1VL
Kısa Bilgi
Synonym(s): Restriction Endonuclease Buffer NACRES: NA.53
Market Price
CAS Number: 9011-14-7
Pre-ordered Product
Sigma-Aldrich B8781 Restriction Enzyme Buffer SB 10 × 5X1 VIAL
Synonym(s): Restriction Endonuclease Buffer
NACRES:
NA.53
Sigma-Aldrich B8781 Restriction Enzyme Buffer SB 10 × 5X1 VIAL     
PROPERTIES
grade for molecular biology
description
10× buffer contains 100 mM Tris-HCl, pH 8.0, 50 mM MgCl2, 1000 mM NaCl, 10 mM 2-mercaptoethanol at 37 °C.
form
buffered aqueous solution concentration 10 ×
storage temp. −20°C
SMILES string
CC(C)CC(CNC(Cc1ccccc1)C(N)=O)NC(=O)C(Cc2cnc[nH]2)NC(=O)CNC(=O)C(NC(=O)C(C)NC(=O)
C(Cc3c[nH]c4ccccc34)NC(=O)C(CCC(N)=O)NC(=O)C(N)Cc5c[nH]c6ccccc56)C(C)C InChI 1S/C58H77N15O9/c1-32(2)21-39(29-65-46(52(61)76)
22-35-13-7-6-8-14-35)69-57(81)48(25-38-28-62-31-67-38)70-50(75)30-66-58(82)51(33(3)4)73-53(77)34(5)68-56(80)47(24-37-27-64-44-18-12-10-16-41(37)44)72-55(79)45(19-20-49(60)74)71-54(78)42(59)23-36-26-63-43-17-11-9-15-40(36)43/h6-18,26-28,31-34,39,42,45-48,51,63-65H,19-25,29-30,59H2,1-5H3,(H2,60,74)(H2,61,76)(H,62,67)(H,66,82)(H,68,80)(H,69,81)(H,70,75)(H,71,78)(H,72,79)(H,73,77)
InChI key
YNZJHLFHFNCZIE-UHFFFAOYSA-N


DESCRIPTION
General description
This buffer is for use with Sigma Restriction Enzymes. Please note every restriction enzyme is supplied with the appropriate buffer - this product is available for users who need additional buffer. For details on buffer composition and protocols for use, see the "Protocols and Articles" tab above.
Application
Restriction Enzyme Buffer SB has been used in restriction digestion of PCR amplified product.[1]

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Sigma-Aldrich B8781 Restriction Enzyme Buffer SB 10 × 5X1 VIAL
Sigma-Aldrich B8781 Restriction Enzyme Buffer SB 10 × 5X1 VIAL Sigma-Aldrich B8781 Restriction Enzyme Buffer SB 10 ×, Synonym(s): Restriction Endonuclease Buffer NACRES: NA.53 LB.SA.B8781-5X1VL
Sigma-Aldrich B8781 Restriction Enzyme Buffer SB 10 × 5X1 VIAL

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