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Sigma-Aldrich B9754 BIS-TRIS ≥98.0% (titration) CAS No.: 6976-37-0 1 kg
Brand
Stock Code
LB.SA.B9754-1KG
Kısa Bilgi
Synonym(s): 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane Empirical Formula (Hill Notation): C8H19NO5 CAS Number: 6976-37-0 Molecular Weight: 209.24 Beilstein: 2205275 EC Number: 230-237-7 MDL number: MFCD00002853 UNSPSC Code: 12161700 PubChem Substance ID: 24892155 NACRES: NA.25
| Sigma-Aldrich B9754 BIS-TRIS ≥98.0% (titration) CAS No.: 6976-37-0 |
| Synonym(s): 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane Empirical Formula (Hill Notation): C8H19NO5 CAS Number: 6976-37-0 Molecular Weight: 209.24 Beilstein: 2205275 EC Number: 230-237-7 MDL number: MFCD00002853 UNSPSC Code: 12161700 PubChem Substance ID: 24892155 NACRES: NA.25 |
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PROPERTIES
Assay ≥98.0% (titration)
form crystalline powder storage condition dry at room temperature technique(s) UV/Vis spectroscopy: suitable electrophoresis: suitable color white useful pH range 5.8-7.2 p Ka (25 °C) 6.5 solubility H2O: 500 mg/mL, clear, colorless to very faintly yellow suitability suitable for Western blot suitable for column chromatography of proteins application(s) diagnostic assay manufacturing general analytical microbiology SMILES string OCCN(CCO)C(CO)(CO)CO InChI 1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2 InChI key OWMVSZAMULFTJU-UHFFFAOYSA-N DESCRIPTION General description Bis-tris methane, also known as BIS-TRIS or BTM, is a zwitterionic buffering agent widely employed in biochemical and biological research. It functions effectively in aqueous solutions within a pH range of 5.8 to 7.2 and demonstrates minimal shifts in its dissociation constant. Further, it can be used as a substitute for the highly toxic buffer cacodylate and is also recognized for its ability to form complexes with different metals. Application Bis-Tris has been used : - as a buffer for Electrophoresis[1] - as a component of transfer buffer for Western Blotting[2] Features and Benefits Suitable for Biological and Biochemical Research Effective Buffering from pH 5.8-7.2 (25 °C) with a pKa of 6.5 (25 °C) Other Notes For additional information on our range of Biochemicals, please complete this form. |
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