Sigma-Aldrich B9754 BIS-TRIS ≥98.0% (titration) CAS No.: 6976-37-0 1 kg

Category

Moleküler Biyoloji ve Biyokimya Ürünleri , Diğer Analitik Kimyasallar

Brand

SIGMA-ALDRICH

Stock Code

LB.SA.B9754-1KG

Kısa Bilgi

Synonym(s): 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane Empirical Formula (Hill Notation): C8H19NO5 CAS Number: 6976-37-0 Molecular Weight: 209.24 Beilstein: 2205275 EC Number: 230-237-7 MDL number: MFCD00002853 UNSPSC Code: 12161700 PubChem Substance ID: 24892155 NACRES: NA.25

Teklif Sepetine Ekle

Stok Sorunuz

Sigma-Aldrich B9754 BIS-TRIS ≥98.0% (titration) CAS No.: 6976-37-0

Synonym(s): 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
Empirical Formula (Hill Notation): C₈H₁₉NO₅
CAS Number: 6976-37-0
Molecular Weight: 209.24
Beilstein: 2205275
EC Number: 230-237-7
MDL number: MFCD00002853
UNSPSC Code: 12161700
PubChem Substance ID: 24892155
NACRES: NA.25

PROPERTIES

Assay ≥98.0% (titration)
form crystalline powder
storage condition dry at room temperature
technique(s)
UV/Vis spectroscopy: suitable
electrophoresis: suitable color white useful
pH range 5.8-7.2 p
Ka (25 °C) 6.5
solubility H₂O: 500 mg/mL, clear, colorless to very faintly yellow
suitability
suitable for Western blot
suitable for column chromatography of proteins
application(s)
diagnostic assay manufacturing
general analytical
microbiology
SMILES string OCCN(CCO)C(CO)(CO)CO
InChI 1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
InChI key OWMVSZAMULFTJU-UHFFFAOYSA-N

DESCRIPTION
General description
Bis-tris methane, also known as BIS-TRIS or BTM, is a zwitterionic buffering agent widely employed in biochemical and biological research. It functions effectively in aqueous solutions within a pH range of 5.8 to 7.2 and demonstrates minimal shifts in its dissociation constant. Further, it can be used as a substitute for the highly toxic buffer cacodylate and is also recognized for its ability to form complexes with different metals.
Application
Bis-Tris has been used :
- as a buffer for Electrophoresis^[1]
- as a component of transfer buffer for Western Blotting^[2]
Features and Benefits
Suitable for Biological and Biochemical Research Effective Buffering from pH 5.8-7.2 (25 °C) with a pKa of 6.5 (25 °C)
Other Notes
For additional information on our range of Biochemicals, please complete this form.

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