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Sigma-Aldrich D8418 Dimethyl sulfoxide for molecular biology 1 L
Brand
Stock Code
LB.SA.D8418-1L
| Sigma-Aldrich D8418 Dimethyl sulfoxide for molecular biology 1 L |
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Synonym(s): Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO
Linear Formula: (CH3)2SO
CAS Number: 67-68-5
Molecular Weight: 78.13
Beilstein: 506008
EC Number: 200-664-3
MDL number: MFCD00002089
PubChem Substance ID: 24894214
NACRES: NA.52
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PROPERTIES
grade for molecular biology
Quality Level 200
vapor density 2.7 (vs air)
vapor pressure 0.42 mmHg (20 °C)
product line BioReagent
assay ≥99.9%
form liquid
autoignition temp. 573 °F
shelf life Recommended retest period - 2 years
expl. lim. 42 %, 63 °F
technique(s)
DNA sequencing: suitable
PCR: suitable
transfection: suitable
impurities
≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)
color colorless
refractive index n20/D 1.479 (lit.)
bp 189 °C (lit.)
mp 16-19 °C (lit.)
solubility H2O: miscible (completely)
density 1.10 g/mL (lit.)
absorption passes test
suitability suitable for molecular biology
foreign activity DNase and RNase, none detected
storage temp. room temp
SMILES string CS(C)=O
InChI 1S/C2H6OS/c1-4(2)3/h1-2H3
InChI key IAZDPXIOMUYVGZ-UHFFFAOYSA-N
DESCRIPTION
General description Features and Benefits: - Suitable for Molecular Applications
- Nuclease-Free DMSO
- DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
- Molecular Biology Grade DMSO
Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals. This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.
Application DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many
applications in organic chemistry and molecular biology. DMSO is routinely used in
- polymerase chain reaction (PCR)
- amplification of cDNA libraries
- DNA sequencing
- column-loading
- buffers for poly(A)+ RNA selection buffers for the
- transformation of competent E. coli
- transfection protocols.
Dimethyl sulfoxide has been used-
as an oligonucleotide solvent in array spotting[1]
as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.
Packaging 50, 100, 250, 500 mL in glass bottle
1 L in glass bottle
Features and Benefits This product is free from DNases, RNases, phosphatases and proteases.
Caution Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.
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