Sigma-Aldrich E1510 Ethidium bromide solution BioReagent, for molecular biology, 10 mg/mL in H2O 10 mL
Brand
Stock Code
LB.SA.E1510-10ML
Kısa Bilgi
Synonym(s): 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide Empirical Formula (Hill Notation): C21H20BrN3 CAS Number: 1239-45-8 Molecular Weight: 394.31 Beilstein: 3642536 MDL number: MFCD00011724 PubChem Substance ID: 24894418 NACRES: NA.31
| Sigma-Aldrich E1510 Ethidium bromide solution BioReagent, for molecular biology, 10 mg/mL in H2O |
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Synonym(s): 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide
Empirical Formula (Hill Notation): C21H20BrN3
CAS Number: 1239-45-8
Molecular Weight: 394.31
Beilstein: 3642536
MDL number: MFCD00011724
PubChem Substance ID: 24894418
NACRES: NA.31
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PROPERTIES
grade for molecular biology
Quality Level 200
product line BioReagent
concentration 10 mg/mL in H2O
technique(s) electrophoresis: suitable
suitability suitable for gel electrophoresis
SMILES string [Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
InChI 1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H
InChI key ZMMJGEGLRURXTF-UHFFFAOYSA-N
DESCRIPTION
Application
Ethidium bromide solution has been used: as a stain to visualize U937 cells to assess cell viability[1]
to detect polymerase chain reaction products[2]
in agarose gel electrophoresis based gel retardation assay[3]
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.
Packaging
10 mL in glass bottle
Biochem/physiol Actions
Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
Reconstitution
For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.
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