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Sigma-Aldrich H3506 Hyaluronidase from bovine testes Type I-S, lyophilized powder, 400-1000 units/mg solid 100 mg
Brand
Stock Code
LB.SA.H3506-100MG
Kısa Bilgi
Synonym(s): Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase CAS Number: 37326-33-3 Enzyme Commission number: 3.2.1.35 (BRENDA, IUBMB) EC Number: 253-464-3 MDL number: MFCD00131351 NACRES: NA.54
Warranty Period
24 Month
Market Price
AS Number: 37326-33-3
| Sigma-Aldrich H3506 Hyaluronidase from bovine testes Type I-S 100 mg |
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Synonym(s): Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
CAS Number: 37326-33-3
Enzyme Commission number: 3.2.1.35 (BRENDA, IUBMB)
EC Number: 253-464-3
MDL number: MFCD00131351
NACRES: NA.54
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PROPERTIES
type Type I-S
Quality Level 200
form lyophilized powder
specific activity 400-1000 units/mg solid
mol wt ~55 kDa (four subunits of 14 kDa each)
technique(s) cell culture | mammalian: suitable, single cell analysis: suitable
UniProt accession no. Q5E985 Q8SQG8
application(s) diagnostic assay manufacturing
shipped in wet ice
storage temp. −20°C
DESCRIPTION
Application
Hyaluronidase from bovine testes has been used for the digestion of breast tissue, heart tissue and oocytes for isolation of cells.[1][2][3]
Suitable for use in preparation of single cell suspension for sequencing.
Biochem/physiol Actions
Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression. These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Unit Definition
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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