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Sigma-Aldrich MAK461 pNPP Phosphatase Assay Kit Sufficient for 500 colorimetric tests
| Sigma-Aldrich MAK461 pNPP Phosphatase Assay Kit Sufficient for 500 colorimetric tests |
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EC Number: 245-325-0
eCl@ss: 42010105
NACRES: NA.53
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| PROPERTIES
Quality Level 100
detection method colorimetric
storage temp. −20 °C
DESCRIPTION
General description
Para-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at A405 nm on a spectrophotometer.
Features and Benefits
No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes.
Compatible with high-throughput handling systems.
Suitability
The kit is suitable for the quantitative determination of phosphatase activity of protein phosphatases.
Principle
The pNPP Phosphatase Assay kit involves adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37 °C. |