Homepage
Mikrobiyoloji
Epigenetics
ZYMO RESEARCH D5003 EZ-96 DNA Methylation Kit (Shallow-Well) 2 x 96 Rxns

ZYMO RESEARCH D5003 EZ-96 DNA Methylation Kit (Shallow-Well) 2 x 96 Rxns

Category

Epigenetics

Brand

ZYMO RESEARCH

Stock Code

LB.MB.ZYMD5003

Sepete Ekle

Teklif Sepetine Ekle

ZYMO RESEARCH D5003 EZ-96 DNA Methylation Kit (Shallow-Well) 2 x 96 Rxns

Streamlined, proven procedure for bisulfite conversion of DNA.

Desulphonation and recovery of bisulfite-treated DNA with a 96-well spin-column plate.

Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.

The EZ-96 DNA Methylation Kits are bisulfite conversion kits that feature a high-throughput (96-well spin-plate), simplified procedure that streamlines bisulfite conversion of DNA. Cytosines undergo a three-step reaction with sodium bisulfite during which the cytosine is converted into uracil. The innovative in-column desulphonation technology eliminates otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.

Applications	Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # D5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion	>99%
Elution Volume	≥ 15 µl for Deep-well ≥ 30 µl for Shallow-well
Equipment	Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input	500 pg - 2 µg of DNA
Processing Time	12-16 hours
Recovery	>80%
Sample Source	Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
Supplemental Info	Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link

Taksit Seçenekleri

Önerileriniz

You can use the suggestion form to submit feedback on the product's price, image, description, or any other insufficient areas.
Thank you for your feedback and suggestions.

Product image is poor quality, corrupted, or not viewable.

Missing information in the product description.

Errors in product information.

Product is more expensive than on other sites.

There should be other alternatives to this product.

ZYMO RESEARCH D5003 EZ-96 DNA Methylation Kit (Shallow-Well) 2 x 96 Rxns

Recommend