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ZYMO RESEARCH D5007 EZ-96 DNA Methylation-Gold Kit (Shallow-Well) 2 x 96 Rxns

ZYMO RESEARCH D5007 EZ-96 DNA Methylation-Gold Kit (Shallow-Well) 2 x 96 Rxns

ZYMO RESEARCH D5007 EZ-96 DNA Methylation-Gold Kit (Shallow-Well) 2 x 96 Rxns

Category

Epigenetics

Brand

ZYMO RESEARCH

Stock Code

LB.MB.ZYMD5007

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ZYMO RESEARCH D5007 EZ-96 DNA Methylation-Gold Kit (Shallow-Well) 2 x 96 Rxns

High-throughput (96-well) bisulfite conversion of DNA in less than 3 hours.

A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines into uracil.

Desulphonation and recovery of bisulfite-treated DNA with a 96-well plate.

Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.

The EZ-96 DNA Methylation-Gold Kit allows high-throughput (96-well spin-plate) conversion of unmethylated cytosines into uracil in less than 3 hours. Our optimized bisulfite chemistry combines heat denaturation and bisulfite conversion of input DNA to reduce incubation time and ensure conversion rates >99%. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc

Applications	Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. Catalog # D5004 is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
Conversion	>99%
Elution Volume	≥ 15 µl for Deep-well ≥ 30 µl for Shallow-well
Equipment	Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers
Input	500 pg - 2 µg of DNA.
Processing Time	3 hours
Recovery	>75%
Sample Source	Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
Supplemental Info	Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link

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ZYMO RESEARCH D5007 EZ-96 DNA Methylation-Gold Kit (Shallow-Well) 2 x 96 Rxns

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