Negative control for epigenetic experiments requiring DNA containing zero background levels of cytosine and adenosine methylation. Substrate for DNA methylation-sensitive restriction enzyme digestions.

E. coli non-methylated genomic DNA is from a Dam– and Dcm– strain (ER2925) of E. coli. It works perfectly as a negative control for DNA methylation analyses requiring DNA with absolutely no methylation.

Concentration	250 ng/µl in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)
Genotype	ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2
Storage	≤ -20°C
Supplemental Info	Controls for DNA Methylation Assays

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ZYMO RESEARCH D5016 E. coli Non-Methylated Genomic DNA 5 µg/20 µl

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