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ZYMO RESEARCH D5030-E EZ DNA Methylation-Lightning Kit (CE-IVD) 50 Rxns

ZYMO RESEARCH D5030-E EZ DNA Methylation-Lightning Kit (CE-IVD) 50 Rxns

ZYMO RESEARCH D5030-E EZ DNA Methylation-Lightning Kit (CE-IVD) 50 Rxns

Category

Epigenetics

Brand

ZYMO RESEARCH

Stock Code

LB.MB.ZYMD5030-E

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ZYMO RESEARCH D5030 EZ DNA Methylation-Lightning Kit (CE-IVD) 50 Rxns

Fastest bisulfite conversion kit for complete bisulfite conversion of DNA for methylation analysis.

Ready-to-use conversion reagent is added directly to DNA.

High-yield, converted DNA is ideal for PCR, Methylation Specific PCR (MSP), arrays, library preps, Next-Generation sequencing, etc.

ZYMO RESEARCH D5030 EZ DNA Methylation-Lightning Kit (CE-IVD) 50 Rxns

The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process – simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 µl. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.

Applications	Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion	> 99.5%
Elution Volume	Deep-well: ≥ 15 µl Shallow-well: ≥ 30 µl
Equipment	Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input	100 pg - 2 µg of DNA.
Processing Time	1.5 hours
Recovery	> 80%
Sample Source	Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
Supplemental Info	Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link

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ZYMO RESEARCH D5030-E EZ DNA Methylation-Lightning Kit (CE-IVD) 50 Rxns

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