ZYMO RESEARCH D5033 EZ-96 DNA Methylation-Lightning Kit (Deep-Well) 2 x 96 Rxns.

Category

Epigenetics

Brand

ZYMO RESEARCH

Stock Code

LB.MB.ZYMD5033

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ZYMO RESEARCH D5033 EZ-96 DNA Methylation-Lightning Kit (Deep-Well) 2 x 96 Rxns.

Fastest 96-well bisulfite conversion kit for complete, high-throughput (96-well) bisulfite conversion of DNA for methylation analysis.

Ready-to-use conversion reagent is added directly to DNA.

High-yield, converted DNA is ideal for PCR, Methylation Specific PCR (MSP), arrays, library preps, Next-Generation sequencing, etc.

The EZ-96 DNA Methylation-Lightning Kit is a rapid, high-throughput (96-well) bisulfite conversion kit for DNA methylation analysis. The streamlined workflow features ready-to-use Lightning Conversion Reagent and a combined DNA denaturation and bisulfite conversion process. Desulphonation and clean-up of the converted DNA is performed using a unique 96-well spin-plate. With the Deep-Well Kit (Cat. D5033), samples can be eluted in as little as 15 µl. High yield, converted DNA is ideal for PCR, array, and Next-Generation sequencing, etc.

Applications	Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion	> 99.5%
Elution Volume	Deep-well: ≥ 15 µl Shallow-well: ≥ 30 µl
Equipment	Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input	100 pg - 2 µg of DNA.
Processing Time	1.5 hours
Recovery	> 80%
Sample Source	Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
Supplemental Info	Bisulfite Beginner Guide Controls for DNA Methylation Assays Bisulfite Conversion for Illumina Methylation Assays Bisulfite Primer Design Tool Link

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ZYMO RESEARCH D5033 EZ-96 DNA Methylation-Lightning Kit (Deep-Well) 2 x 96 Rxns.

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