ZYMO RESEARCH D5311 OneStep qMethyl-Lite Lite

Category

Epigenetics

Brand

ZYMO RESEARCH

Stock Code

LB.MB.ZYMD5311

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ZYMO RESEARCH D5311 OneStep qMethyl-Lite Lite

Single step, real-time PCR procedure for bisulfite-free determination of DNA methylation status

Includes reagents and controls for quantitative detection and reliable performance

Ideal for rapid screening of single and multi-locus DNA methylation

The OneStep qMethyl Kit provides a simple, straightforward, and bisulfite-free procedure for rapid, locus-specific DNA methylation assessment via the selective amplification of a methylated region of DNA. This is accomplished by splitting any DNA to be tested into two parts: a "Test Reaction” and a "Reference Reaction”. DNA in the Test Reaction is digested with Methylation Sensitive Restriction Enzymes (MSREs) while DNA in the Reference Reaction is not. The DNA from both samples is then amplified using real-time PCR in the presence of SYTO 9 fluorescent dye and quantitated using a simple equation.

Input	High quality DNA suitable for use in restriction enzyme digestion. Require A260/280 g&t; 1.8, A260/230 > 1.8.
Processing Time	4.5 hours
Storage	Store at -20°C for up to 12 months. Avoid repeated freeze-thaw cycles of reagents. Prolong storage at -80°C. 2X Test Reaction PreMix and 2X Reference Reaction PreMix are light sensitive. Minimize light exposure.

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ZYMO RESEARCH D5311 OneStep qMethyl-Lite Lite

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