ZYMO RESEARCH D5325 5-mC DNA ELISA Kit 1 x 96 Preps

Category

Epigenetics

Brand

ZYMO RESEARCH

Stock Code

LB.MB.ZYMD5325

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ZYMO RESEARCH D5325 5-mC DNA ELISA Kit 1 x 96 Preps

For high-throughput detection of global 5-methylcytosine (5-mC) in DNA

The streamlined workflow can be completed in less than 3 hours.

The 5-mC DNA ELISA Kit allows researchers to accurately quantitate 5-mC in any DNA sample in less than 3 hours. The kit features an Anti-5-Methylcytosine monoclonal antibody that is both sensitive and specific for 5-mC. The assay is compatible with a wide range of input DNA from vertebrate, plant, and microbial sources as well as methylated PCR amplicons and fragmented DNA. Percent 5-mC in a DNA sample can be accurately quantified from a standard curve generated with specially designed controls included with the kit. Also, the fast, streamlined workflow is ideal for high-throughput analyses.

Detection	> 0.5% 5-mC per 100 ng DNA
Equipment	Incubator and ELISA plate reader. A multi-channel pipettor is recommended. An automated plate washer may be used for blocking and wash steps.
Input	This protocol is optimized for 100 ng input DNA/well. Compatible with DNA in the range of 10-200 ng/well.
Sample Source	Purified genomic DNA, plasmid DNA, PCR amplification products, or DNA fragments in water, Tris-EDTA, or similar.

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ZYMO RESEARCH D5325 5-mC DNA ELISA Kit 1 x 96 Preps

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