Quick and simple procedure for completely degrading DNA into its individual nucleotide component for quantitative analysis (e.g., whole-genome methylation analysis by HPLC, TLC, etc.).

1 hour, single-enzyme digest vs. conventional 6 - 16-hour multi-step enzyme digestion protocols

DNA Degradase from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleotide components. DNA Degradase is ideal for whole-genome DNA methylation analysis by many downstream applications (i.e., HPLC, TLC, etc.). Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.

Assay Condition	DNA Degradase in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37 °C for ≥ 1 hour.
Concentration	10 U/µl
Enzyme Inactivation	70 °C for 20 minutes
Storage	Store at -20 °C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70 °C.
Unit Definition	One unit (U) is the amount of enzyme required to degrade 1 µg of λ

ZYMO RESEARCH E2030 Atlantis dsDNase 12.5 U

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ZYMO RESEARCH E2017 DNA Degradase 2000 U

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