Fragments generated using dsDNA Shearase Plus are ideal for library construction, Next-Gen sequencing, methylated DNA immunopreciptation (MeDIP, MeDIP-Seq), etc.

dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs, thus minimizing sample loss.

Concentration	1 U/µl
Enzyme Inactivation	Heat inactivate enzyme at 65 °C for 5 min.
Storage	Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70°C.
Unit Definition	One unit (1 U) is defined at the amount of enzyme required to convert 250 ng human DNA into DNA fragments in the range of 100-500 bp in 20 minutes at 42°C in total reaction volume in 10 µl.

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ZYMO RESEARCH E2018 dsDNA Shearase Plus 200 U

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