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Sigma-Aldrich M3148 2-Mercaptoethanol for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) CAS. No;60-24-2 250 mL
Brand
Stock Code
LB.SA.M3148.0250
Kısa Bilgi
Synonym(s): β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol Linear Formula: HSCH2CH2OH CAS Number: 60-24-2 Molecular Weight: 78.13 Beilstein: 773648 EC Number: 200-464-6 MDL number: MFCD00004890 PubChem Substance ID: 24896800 NACRES: NA.31
| Sigma-Aldrich M3148 2-Mercaptoethanol for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) |
| Synonym(s): β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol Linear Formula: HSCH2CH2OH CAS Number: 60-24-2 Molecular Weight: 78.13 Beilstein: 773648 EC Number: 200-464-6 MDL number: MFCD00004890 PubChem Substance ID: 24896800 NACRES: NA.31 |
|
PROPERTIES
grade for molecular biology
Quality Level 200 vapor density 2.69 (vs air) vapor pressure 1 mmHg ( 20 °C) product line BioReagent Assay 99% (GC/titration) form liquid expl. lim. 18 % reaction suitability reagent type: reductant concentration 14.3 M (pure liquid) technique(s) cell culture | mammalian: suitable, electrophoresis: suitable refractive index n20/D 1.500 (lit.) pH 4.5-6 (20 °C, 500 g/L) bp 157 °C (lit.) solubility H2O: soluble 1 mL/mL density 1.114 g/mL at 25 °C (lit.) foreign activity DNase, RNase, protease, none detected storage temp. room temp SMILES string OCCS InChI 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2 InChI key DGVVWUTYPXICAM-UHFFFAOYSA-N DESCRIPTION
Application
Reducing agent β-mercaptoethanol or 2-mercaptoethanol has been used: - as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)[1] - to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells[2] - to trypsinize mouse embryonic stem cells[3] BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. |
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