Sigma-Aldrich M6250 2-Mercaptoethanol ≥99.0% CAS No.: 60-24-2 100 mL

Category

Diğer Analitik Kimyasallar , Moleküler Biyoloji ve Biyokimya Ürünleri

Brand

SIGMA-ALDRICH

Stock Code

LB.SA.M6250-100ML

Kısa Bilgi

Synonym(s): β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol Linear Formula: HSCH2CH2OH CAS Number: 60-24-2 Molecular Weight: 78.13 Beilstein: 773648 EC Number: 200-464-6 MDL number: MFCD00004890 PubChem Substance ID: 57654402 NACRES: NA.22

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Sigma-Aldrich M6250 2-Mercaptoethanol ≥99.0% 100 mL

Synonym(s): β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
Linear Formula: HSCH₂CH₂OH
CAS Number: 60-24-2
Molecular Weight: 78.13
Beilstein: 773648
EC Number: 200-464-6
MDL number: MFCD00004890
PubChem Substance ID: 57654402
NACRES: NA.22

PROPERTIES

vapor density 2.69 (vs air)
Quality Level 200
vapor pressure 1 mmHg ( 20 °C)
Assay ≥99.0%
form liquid
expl. lim. 18 %
concentration 14.3 M (pure liquid)
refractive index n20/D 1.500 (lit.)
pH 4.5-6 (20 °C, 500 g/L)
bp 157 °C (lit.)
density 1.114 g/mL at 25 °C (lit.)
storage temp. 2-8°C
SMILES string OCCS
InChI 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
InChI key DGVVWUTYPXICAM-UHFFFAOYSA-N

DESCRIPTION

General description
2-mercaptoethanol is a thiol compound,^[1] commonly used as a reducing agent in organic reactions.^[2]
Application
2-mercaptoethanol is widely used for retarding oxidation of biological compounds in solution. BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structur

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