BOSTER R00121-1 Recombinant Human FGF2 Protein 100 μg/vial
| R00121-1 Recombinant Human FGF2 Protein 100 μg/vial |
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Cat. No: R00121-1
Size: 100 μg
Amino Acid Sequence:PALPE DGGAA FPPGH FKDPK RLYCKNGGFF LRIHP DGRVD GVREK SDPHVKLQLQ AEERG VVSIK GVCANRYLAMKEDGR LLASK CVTEE CFFFE RLESNNYNTY RSRKY SSWYV ALKRT GQYKLGSKTG PGQKA ILFLP MSAKS
Source: E. coli P10-S154
Species: mouse
Purity: >95%, by SDS-PAGE quantitativedensitometry by Coomassie®BlueStaining
Molecular Weight: 16.3KD
Formulation: Lyophilized after extensivedialysis against PBS
Reconstitution: Reconstitute in ddH2Oat
100 μg/mL.
Endotoxin: Less than 1 EU/μg of rmbFGFas determined by LAL metho.
Storage:Lyophilized recombinant Mouse Fibroblast
Growth Factor basic (FGF-basic/FGF-2)
remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmFGF2 remains stable up to 2 weeks at
4 °C or up to 3 months at -20 °C
Description
Fibroblast Growth Factor basic (FGF-basic/FGF-2) is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FGF-1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor. Other homologous FGF belonging to the same family are int-2 (FGF-3), FGF-5, FGF-6, K-FGF and KGF (keratinocyte growth factor = FGF-7). All factors are products of different genes, some of which are Oncogene products (FGF-3, FGF-4, FGF-5). |
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A Recombinant protein is a protein produced artificially by an expression host organism, aka expression system, that is transfected with a recombinant gene (target gene) isolated from another organism. The purpose is to produce the target gene encoded protein in large quantities for medical, research and academic uses. Expression Systems There are several common expression systems for producing recombinant proteins, such as E. Coli, Yeast, Insect Cell lines such as Sf21, mammalian cells such as CHO, HEK, and human cell lines. These expression systems act as the factory for recombinant protein production, where the cellular mechanisms involved in protein production uses the recombinant gene in the vector as blueprint to mass produce the target protein. E. Coli is the first organism to be used as an expression system. It is easy to use however cannot produce protein with post-translational modification (PTM) because it lacks the cellular mechanisms of eukaryotic cells which is necessary for PTM of proteins. Thus for producing simple proteins and for applications that do not require proteins to have PTM, E. Coli is a sufficient and convenient expression system however for more complex applications, more advanced expression systems are required. Expression Vectors A vector is a tool for manipulating DNA. It is the transport vehicle for the gene of the protein of interest. There are many types of vectors, some common vectors are plasmids and reverse transcription viruses. The DNA that encodes the protein is first synthesized in vitro, or cloned from native host DNA templates, then inserted into the vector DNA. The host organism is processed to take up vectors. These vectors, once inside the corresponding host cells, will be transcribed and translated into proteins. |
