MERCK 101406 VRB (Violet Red Bile Lactose) agar acc. ISO 4832 and FDA-BAM GranuCult® 500 gr
Catalogue Number	101406
Replaces	1014065007
Synonyms	Crystal-violet neutral red bile agar, VRB-agar (Crystal-violet neutral-red bile agar)
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Product Information
HS Code	3821 00 00
Quality Level	MQ300

Physicochemical Information
pH value	7.3 - 7.5 (40 g/l, H₂O, 37 °C) (after autoclaving)
Bulk density	670 kg/m3
Solubility	39.5 g/l

Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 1 slightly hazardous to water
Disposal	3 Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Storage and Shipping Information
Storage	Store at +15 °C to +25 °C.

Specifications
Appearance (clearness)	clear
Appearance (colour)	red
pH-value (25 °C)	7.2 - 7.6
Solidification behaviour (2 hrs., 45 °C)	liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))
Colony count (Escherichia coli ATCC 8739 (WDCM 00012))
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))	≥ 50 %
Colony colour (Escherichia coli ATCC 8739 (WDCM 00012))	purplish-red colonies with or without precipitate halo
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))
Colony count (Escherichia coli ATCC 25922 (WDCM 00013))
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013))	≥ 50 %
Colony colour (Escherichia coli ATCC 25922 (WDCM 00013))	purplish-red colonies with or without precipitate halo
Inoculum on reference medium (Enterobacter cloacae ATCC 13047 (WDCM 00083))
Colony count (Enterobacter cloacae ATCC 13047 (WDCM 00083))
Recovery on test medium (Enterobacter cloacae ATCC 13047 (WDCM 00083))	≥ 50 %
Colony colour (Enterobacter cloacae ATCC 13047 (WDCM 00083))	purplish-red colonies with or without precipitate halo
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	no limit
Colony colour (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025))	colourless to beige colonies
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009))	total inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087))	total inhibition
Incubation: 24 ± 2 hours at 30 ± 1 °C Reference media: Tryptic Soy Agar
A recovery rate of 50 % is equivalent to a productivity value of 0.5. The indicated colony counts result from the sum of a triple determination.

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Merck 101406 Vrb (Violet Red Bile Lactose) Agar Acc. Iso 4832 And Fda-Bam granucult® 500 gr

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