Description
Catalogue Number	115940
Synonyms	Hexamethylenepararosaniline chloride, Gentian violet (C.I. 42555), Hexamethyl-p-rosanilinium, Methyl violet 10 B
Description	CRYSTAL VIOLET (C.I. 42555) FOR MICROSCO
Overview	Crystal violet (C.I. 42555) is a CERTISTAIN® dry dye that can be used to produce a Crystal violet staining solution to differentiate Gram-positive and Gram-negative bacteria in samples of human origin. In the case of Gram-positive bacteria, the bacteria retain their dark blue / dark purple color even after the treatment with decolorizing solution. On the other side Gram-negative bacteria are stained red or pink with the counterstain. The CERTISTAIN® dry dye can be used for clinical diagnostic purposes as it is certified and registered as IVD and CE product.

Product Information
CAS number	548-62-9
EC index number	612-204-00-2
EC number	208-953-6
Hill Formula	C₂₅H₃₀ClN₃
Molar Mass	407.99 g/mol
HS Code	3204 13 00
Structure formula Image

Applications
Application	Crystal violet (C.I. 42555) for microscopy Certistain®. CAS 548-62-9, pH 2.5 - 3.5 (10 g/l, H₂O, 20 °C).

Physicochemical Information
Density	1.19 g/cm3 (20 °C)
Melting Point	189 - 194 °C
pH value	2.5 - 3.5 (10 g/l, H₂O, 20 °C)
Bulk density	220 - 400 kg/m3
Solubility	10 g/l

Toxicological Information
LD 50 oral	LD50 Rat 420 mg/kg

Safety Information according to GHS
Hazard Pictogram(s)
Hazard Statement(s)	H302: Harmful if swallowed. H318: Causes serious eye damage. H351: Suspected of causing cancer. H410: Very toxic to aquatic life with long lasting effects.
Precautionary Statement(s)	P273: Avoid release to the environment. P280: Wear eye protection. P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
RTECS	BO9000000
Storage class	10 - 13 Other liquids and solids
WGK	WGK 3 highly water endangering
Disposal	9 Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).

Safety Information
Hazard Symbols	Harmful Dangerous for the environment
Categories of danger	harmful, carcinogenic, irritant, dangerous for the environment
R Phrase	R 22-40-41-50/53 Harmful if swallowed.Limited evidence of a carcinogenic effect.Risk of serious damage to eyes.Very toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
S Phrase	S 26-36/37/39-46-60-61 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.Wear suitable protective clothing, gloves and eye/face protection.If swallowed, seek medical advice immediately and show this container or label.This material and its container must be disposed of as hazardous waste.Avoid release to the environment. Refer to special instructions/ Safety data sheets.

Storage and Shipping Information
Storage	Store at +5°C to +30°C.

Transport Information
Declaration (railroad and road) ADR, RID	UN 3077 , 9, III
Declaration (transport by air) IATA-DGR	UN 3077 , 9, III
Declaration (transport by sea) IMDG-Code	UN 3077 , 9, III, Marine Pollutant: P

Specifications
Dye content (spectrophotometrically)	≥ 88 %
Identity (UV/VIS-Spectrum)	passes test
Absorption maximum λ max (water)	589 - 594 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.002 g/l; water)	2050 - 2450
TLC-Test	passes test
Michler`s ketone	< 0.10 %
Loss on drying (110 °C)	≤ 10 %
Suitability for microscopy	passes test

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