BOSTER R00393 Recombinant Human IFN Gamma Protein 100 μg/vial
| R00393 Recombinant Human IFN Gamma Protein 100 μg/vial |
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Cat. No: R00393
Size: 100
Amino Acid Sequence:QDPYV KEAEN LKKYF NAGHSDVADNGTLFL GILKN WKEES DRKIMQSQIVSFYFK LFKNF KDDQS IQKSVETIKEDMNVK FFNSN KKKRD DFEKL TNYSVTDLNV QRKAI HELIQ VMAEL SPAAKTGKRK RSQML FRG
Source: E. coli Gln24-Gly161
Species: human
Purity: >95%, by SDS-PAGE quantitativedensitometry by Coomassie® BlueStaining.
Molecular Weight: 16.1KD
Formulation: Lyophilized after extensivedialysis against PBS
Reconstitution: Reconstitute in ddH2Oat
100 μg/mL
Endotoxin: Less than 1 EU/μg of IFNγas determined by LAL method
Storage:Lyophilized recombinant human Interferon gamma remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhIFNγ remains stable upto2 weeks at 4 °C or up to 3 months at -20 °C
Description
Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. The existence of this interferon, which early in its history was known as immune interferon, was described by E. F. Wheelock as a product of human leukocytes stimulated with phytohemagglutinin, and by others as a product of antigen-stimulated lymphocytes or tuberculin-sensitized mouse peritoneal lymphocytes challenged with PPD. The resulting supernatants were shown to inhibit growth of vesicular stomatitis virus. IFNγ is a cytokine that is critical for innate and adaptive immunity against viral, some bacterial and protozoal infections. IFNγ is an important activator of macrophages and inducer of Class II major histocompatibility complex (MHC) molecule expression. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNγ is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops. |
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A Recombinant protein is a protein produced artificially by an expression host organism, aka expression system, that is transfected with a recombinant gene (target gene) isolated from another organism. The purpose is to produce the target gene encoded protein in large quantities for medical, research and academic uses. Expression Systems There are several common expression systems for producing recombinant proteins, such as E. Coli, Yeast, Insect Cell lines such as Sf21, mammalian cells such as CHO, HEK, and human cell lines. These expression systems act as the factory for recombinant protein production, where the cellular mechanisms involved in protein production uses the recombinant gene in the vector as blueprint to mass produce the target protein. E. Coli is the first organism to be used as an expression system. It is easy to use however cannot produce protein with post-translational modification (PTM) because it lacks the cellular mechanisms of eukaryotic cells which is necessary for PTM of proteins. Thus for producing simple proteins and for applications that do not require proteins to have PTM, E. Coli is a sufficient and convenient expression system however for more complex applications, more advanced expression systems are required. Expression Vectors A vector is a tool for manipulating DNA. It is the transport vehicle for the gene of the protein of interest. There are many types of vectors, some common vectors are plasmids and reverse transcription viruses. The DNA that encodes the protein is first synthesized in vitro, or cloned from native host DNA templates, then inserted into the vector DNA. The host organism is processed to take up vectors. These vectors, once inside the corresponding host cells, will be transcribed and translated into proteins. |
