BOSTER R00609 Recombinant Human CNTF Protein 100 μg/vial
| R00609 Recombinant Human CNTF Protein 100 μg/vial |
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Cat. No: R00609
Size: 100 μg
Amino Acid Sequence:
HRRDL CSRSI WLARK IRSDL TALTESYVKH QGLNK NINLD SADGMPVASTDQWSE LTEAE RLQEN LQAYRTFHVLLARLL EDQQV HFTPT EGDFHQAIHTLLLQV AAFAY QIEEL MILLE YKIPRNEADG MPINV GDGGL FEKKL WGLKVLQELS QWTVR SIHDL RFISS HQTGI
PARGS HYIAN NKKM
Source: E. coli His12-Met200
Species: human
Purity: >95%, by SDS-PAGE quantitativedensitometry by Coomassie®BlueStaining
Molecular Weight: 21.7KD
Formulation: Lyophilized after extensivedialysis against PBS
Reconstitution: Reconstitute in ddH2Oat
100 μg/mL
Endotoxin: Less than 1 EU/μg of rhCNTFas determined by LAL method
Storage:Lyophilized recombinant human CiliaryNeurotrophic Factor (rhCNTF)remainsstable up to 6 months at -80 °C fromdateof receipt. Upon reconstitution, rhCNTFremains stable up to 2 weeks at 4 °Cor upto 3 months at -20 °C
Description
Ciliary Neurotrophic Factor (CNTF) is a cytokine belonging to the Interleukin 6 (IL-6) family, which also includes IL-6, Oncostatin M, Leukemia Inhibitory Factor (LIF), and Cardiotrophin-1. Structurally, CNTF resembles a four-helix bundle composition, similar to the other members of the IL-6 family. The receptor for CNTF is composed of three parts: a gp130-like subunit common in the IL-6 receptor family, a LIF Receptor β subunit, and a CNTF specific α receptor subunit. Upon binding to the CNTF, the β subunit of the CNTF receptor will undergo tyrosine phosphorylation, and activate the intracellular JAK/STAT pathway. The main function of CNTF in vivo is to promote the differentiation and survival of a variety of neurons and glial cells, including sympathetic precursor cells and spinal motor neurons. Recombinant human Ciliary Neurotrophic Factor (rhCNTF) produced in E. coli is a single non-glycosylated polypeptide chain containing 199 amino acids. A fully biologically active molecule, rhCNTF has a molecular mass of 22.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
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A Recombinant protein is a protein produced artificially by an expression host organism, aka expression system, that is transfected with a recombinant gene (target gene) isolated from another organism. The purpose is to produce the target gene encoded protein in large quantities for medical, research and academic uses. Expression Systems There are several common expression systems for producing recombinant proteins, such as E. Coli, Yeast, Insect Cell lines such as Sf21, mammalian cells such as CHO, HEK, and human cell lines. These expression systems act as the factory for recombinant protein production, where the cellular mechanisms involved in protein production uses the recombinant gene in the vector as blueprint to mass produce the target protein. E. Coli is the first organism to be used as an expression system. It is easy to use however cannot produce protein with post-translational modification (PTM) because it lacks the cellular mechanisms of eukaryotic cells which is necessary for PTM of proteins. Thus for producing simple proteins and for applications that do not require proteins to have PTM, E. Coli is a sufficient and convenient expression system however for more complex applications, more advanced expression systems are required. Expression Vectors A vector is a tool for manipulating DNA. It is the transport vehicle for the gene of the protein of interest. There are many types of vectors, some common vectors are plasmids and reverse transcription viruses. The DNA that encodes the protein is first synthesized in vitro, or cloned from native host DNA templates, then inserted into the vector DNA. The host organism is processed to take up vectors. These vectors, once inside the corresponding host cells, will be transcribed and translated into proteins. |
