BOSTER R00804 Recombinant Human OSM Protein 100 μg/vial
| R00804 Recombinant Human OSM Protein 100 μg/vial |
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Cat. No: R00804
Size: 100 μg
Amino Acid Sequence:AAIGS CSKEY RVLLG QLQKQTDLMQDTSRL LDPYI RIQGL DVPKL REHCRERPGA FPSEE TLRGL GRRGF LQTLNATLGC VLHRL ADLEQ RLPKA QDLERSGLNI EDLEK LQMAR PNILGLRNNI
YCMAQ LLDNS DTAEP TKAGRGASQPPTPTP ASDAF QRKLE GCRFL HGYHRFMHSV GRVFS KWGES PNRSR
Source: E. coli A26-R221
Species: human
Purity: >95%, by SDS-PAGE quantitativedensitometry by Coomassie®BlueStaining.
Molecular Weight: 22KD
Formulation: Lyophilized after extensivedialysis against PBS.
Reconstitution: Reconstitute in ddH2Oat
100 μg/mL.
Endotoxin: Less than 1 EU/μg of OSMas determined by LAL method.
Storage:Lyophilized recombinant humanOncostatin-M remains stable up to6months at -80°C from date of receipt. Upon reconstitution, OSMremains stableup to 2 weeks at 4 °C or up to 3 months at -20 °C.
Description
Oncostatin-M(OSM) is a member of the leukemia inhibitory factor/oncostatin-M (LIF/OSM) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein is a secreted cytokine and growth regulator that inhibits the proliferation of a number of tumor cell lines. This protein also regulates the production of other cytokines, including interleukin 6, granulocyte-colony stimulating factor and granulocyte-macrophage colony stimulating factor in endothelial cells. This gene and the related gene, leukemia inhibitory factor, also present on chromosome 22, may have resulted from the duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. |
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A Recombinant protein is a protein produced artificially by an expression host organism, aka expression system, that is transfected with a recombinant gene (target gene) isolated from another organism. The purpose is to produce the target gene encoded protein in large quantities for medical, research and academic uses. Expression Systems There are several common expression systems for producing recombinant proteins, such as E. Coli, Yeast, Insect Cell lines such as Sf21, mammalian cells such as CHO, HEK, and human cell lines. These expression systems act as the factory for recombinant protein production, where the cellular mechanisms involved in protein production uses the recombinant gene in the vector as blueprint to mass produce the target protein. E. Coli is the first organism to be used as an expression system. It is easy to use however cannot produce protein with post-translational modification (PTM) because it lacks the cellular mechanisms of eukaryotic cells which is necessary for PTM of proteins. Thus for producing simple proteins and for applications that do not require proteins to have PTM, E. Coli is a sufficient and convenient expression system however for more complex applications, more advanced expression systems are required. Expression Vectors A vector is a tool for manipulating DNA. It is the transport vehicle for the gene of the protein of interest. There are many types of vectors, some common vectors are plasmids and reverse transcription viruses. The DNA that encodes the protein is first synthesized in vitro, or cloned from native host DNA templates, then inserted into the vector DNA. The host organism is processed to take up vectors. These vectors, once inside the corresponding host cells, will be transcribed and translated into proteins. |
