BOSTER R01400-1 Recombinant Mouse LIF Protein 100 μg/vial
| R01400-1 Recombinant Mouse LIF Protein 100 μg/vial |
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Cat. No: R01400-1
Size: 100 μg
Amino Acid Sequence:SPLPI TPVNA TCAIR HPCHGNLMNQIKNQL AQLNG SANAL FISYY TAQGEPFPNN VEKLC APNMT DFPSF HGNGTEKTKL VELYR MVAYL SASLT NITRDQKVLN PTAVS LQVKL NATIDVMRGLLSNVL CRLCN KYRVG HVDVP PVPDHSDKEA FQRKK LGCQL LGTYK QVISVVVQAF
Source: E. coli Ser24-Phe203
Species: mouse
Purity: >95%, by SDS-PAGEquantitativedensitometry by Coomassie® Blue Staining.
Molecular Weight: 19.8KD
Formulation: Lyophilized after extensivedialysis against PBS.
Reconstitution: Reconstitute in ddH2Oat 100μg/mL.
Endotoxin: Less than 1 EU/μg of LIFasdetermined by LAL method.
Storage:Lyophilized recombinant Mouse LeukemiaInhibitory Factor remains stable upto6months at -80 °C from date of receipt. Uponreconstitution, rmLIF remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
Description
LIF (leukemia inhibitory factor) is a widely expressed pleiotropic member of the IL-6 family of cytokines. The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene |
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A Recombinant protein is a protein produced artificially by an expression host organism, aka expression system, that is transfected with a recombinant gene (target gene) isolated from another organism. The purpose is to produce the target gene encoded protein in large quantities for medical, research and academic uses. Expression Systems There are several common expression systems for producing recombinant proteins, such as E. Coli, Yeast, Insect Cell lines such as Sf21, mammalian cells such as CHO, HEK, and human cell lines. These expression systems act as the factory for recombinant protein production, where the cellular mechanisms involved in protein production uses the recombinant gene in the vector as blueprint to mass produce the target protein. E. Coli is the first organism to be used as an expression system. It is easy to use however cannot produce protein with post-translational modification (PTM) because it lacks the cellular mechanisms of eukaryotic cells which is necessary for PTM of proteins. Thus for producing simple proteins and for applications that do not require proteins to have PTM, E. Coli is a sufficient and convenient expression system however for more complex applications, more advanced expression systems are required. Expression Vectors A vector is a tool for manipulating DNA. It is the transport vehicle for the gene of the protein of interest. There are many types of vectors, some common vectors are plasmids and reverse transcription viruses. The DNA that encodes the protein is first synthesized in vitro, or cloned from native host DNA templates, then inserted into the vector DNA. The host organism is processed to take up vectors. These vectors, once inside the corresponding host cells, will be transcribed and translated into proteins. |
