BOSTER R01931 Recombinant Human FGF7 Protein 100 μg/vial
| R01931 Recombinant Human FGF7 Protein 100 μg/vial |
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Cat. No: R01931
Size: 100 μg
Amino Acid Sequence:CNDMT PEQMA TNVNC SSPERHTRSYDYMEG GDIRV RRLFC RTQWYLRIDKRGKVK GTQEM KNNYN IMEIRTVAVGIVAIK GVESE FYLAM NKEGKLYAKKECNED CNFKE LILEN HYNTYASAKWTHNGG EMFVA LNQKG IPVRGKKTKKEQKTA HFLPM AIT
Source: E. coli C32- T194
Species: human
Purity: >95%, by SDS-PAGE quantitativedensitometry by Coomassie®BlueStaining
Molecular Weight: 18.9KD
Formulation: Lyophilized after extensivedialysis against PBS
Reconstitution: Reconstitute in ddH2Oat
100 μg/mL
Endotoxin: Less than 1 EU/μg of FGF7as determined by LAL method
Storage:Lyophilized recombinant human FGF7remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhFGF7 remains stable up to 2 weeks at
4 °C or up to 3 months at -20 °C
Description
KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. |
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A Recombinant protein is a protein produced artificially by an expression host organism, aka expression system, that is transfected with a recombinant gene (target gene) isolated from another organism. The purpose is to produce the target gene encoded protein in large quantities for medical, research and academic uses. Expression Systems There are several common expression systems for producing recombinant proteins, such as E. Coli, Yeast, Insect Cell lines such as Sf21, mammalian cells such as CHO, HEK, and human cell lines. These expression systems act as the factory for recombinant protein production, where the cellular mechanisms involved in protein production uses the recombinant gene in the vector as blueprint to mass produce the target protein. E. Coli is the first organism to be used as an expression system. It is easy to use however cannot produce protein with post-translational modification (PTM) because it lacks the cellular mechanisms of eukaryotic cells which is necessary for PTM of proteins. Thus for producing simple proteins and for applications that do not require proteins to have PTM, E. Coli is a sufficient and convenient expression system however for more complex applications, more advanced expression systems are required. Expression Vectors A vector is a tool for manipulating DNA. It is the transport vehicle for the gene of the protein of interest. There are many types of vectors, some common vectors are plasmids and reverse transcription viruses. The DNA that encodes the protein is first synthesized in vitro, or cloned from native host DNA templates, then inserted into the vector DNA. The host organism is processed to take up vectors. These vectors, once inside the corresponding host cells, will be transcribed and translated into proteins. |
