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Sigma-Aldrich E7789 Ethylenediaminetetraacetic acid disodium salt solution for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected 100 mL

Stok Kodu
LB.SA.E7889-100ML
Kısa Bilgi
Synonym(s): (Ethylenedinitrilo)tetraacetic acid disodium salt, EDTA disodium salt, EDTA-Na2 Linear Formula: [-CH2N(CH2CO2Na)CH2CO2H]2 CAS Number: 139-33-3 Molecular Weight: 336.21 Beilstein: 3822669 MDL number: MFCD00070672 PubChem Substance ID: 24894660 NACRES: NA.31
Ön Siparişli Ürün
Sigma-Aldrich E7789 Ethylenediaminetetraacetic acid disodium salt solution for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected
Synonym(s): (Ethylenedinitrilo)tetraacetic acid disodium salt, EDTA disodium salt, EDTA-Na2
Linear Formula:
[-CH2N(CH2CO2Na)CH2CO2H]2
CAS Number:
139-33-3
Molecular Weight:
336.21
Beilstein:
3822669
MDL number:
MFCD00070672
PubChem Substance ID:
24894660
NACRES:
NA.31
Sigma-Aldrich E7789 Ethylenediaminetetraacetic acid disodium salt solution for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected 100 mL  
DESCRIPTION
grade for molecular biology
Quality Level
300
sterility
0.2 μm filtered
form liquid
reaction suitability
reagent type: chelator
concentration
0.5 M in H2O
pH
7.5-8.5
foreign activity
DNase, RNase, NICKase and protease, none detected
SMILES string
[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
InChI
1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2
Inchi Key
ZGTMUACCHSMWAC-UHFFFAOYSA-L


DESCRIPTION

General description
Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid. The four carboxylic acid and two amine groups with lone-pair electrons of EDTA can chelate calcium and several other metal ions. Ethylenediaminetetraacetic acid disodium salt solution is a chelating agent suitable for molecular biology applications.
Application
Ethylenediaminetetraacetic acid disodium salt solution has been used:
- in Tris hydrochloric acid (HCl) for digesting proteinase-K to determine the amount of DNA in the corneal sample
- with papain to digest corneal samples for the quantification of glycosaminoglycan (GAG) content
- in pretreatment for detaching epithelial cells grown to 80% confluency
- to prepare stock solutions for lysis buffer
- in the preparation of urea lysis buffer
Features and
Benefits molecular biology grade
Preparation Note
Prepared in 18 megohm water with Molecular Biology Reagent EDTA (E 5134)

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Sigma-Aldrich E7789 Ethylenediaminetetraacetic acid disodium salt solution for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected 100 mL
Sigma-Aldrich E7789 Ethylenediaminetetraacetic acid disodium salt solution for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected 100 mL Linear Formula: [-CH2N(CH2CO2Na)CH2CO2H]2, CAS Number: 139-33-3, Molecular Weight: 336.21 Beilstein: 3822669, MDL number: MFCD00070672, PubChem Substance ID: 24894660 LB.SA.E7889-100ML
Sigma-Aldrich E7789 Ethylenediaminetetraacetic acid disodium salt solution for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected 100 mL

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