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Sigma-Aldrich S9430 SYBR® Green I nucleic acid gel stain 10,000 x in DMSO 1 mL

Stok Kodu
LB.SA.S9430-1ML
Kısa Bilgi
Synonym(s): DNA stain, SYBR® green gel dye, safer gel stain CAS Number: 163795-75-3 MDL number: MFCD00284715 NACRES: NA.52
Bilgi
CAS Number: 163795-75-3
Sigma-Aldrich S9430 SYBR® Green I nucleic acid gel stain 10,000 x in DMSO 0.5 mL
Synonym(s): DNA stain, SYBR® green gel dye, safer gel stain
CAS Number:
163795-75-3
MDL number:
MFCD00284715
NACRES:
NA.52
Sigma-Aldrich S9430 SYBR® Green I nucleic acid gel stain 10,000 x in DMSO   
PROPERTIES
form solution
Quality Level
200
usage
mL sufficient for 100 mini-gels
greener alternative product
characteristics Designing Safer Chemicals 
concentration
10,000 × in DMSO
technique(s) P
CR: suitable greener alternative category Aligned,
storage temp.
−20°C

DESCRIPTION
General description
SYBR® Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system.[1] SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.[2]
Application
SYBR® Green I nucleic acid gel stain has been used for:
- the quantification of dsDNA[3]
- to stain DNA in polymerase chain reaction (PCR)[4]
- for comet assay technique[5]
- to assess spermatozoon membrane integrity[6]
- for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)[7]
- as a fluorescent dye in flow cytometry[8]
- real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA[8]
Features and Benefits
- An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels
It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps
- It is less mutagenic than ethidium bromide in Ames tests
- It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides
- Useful for many applications with a limited amount of DNA
- The binding of SYBR® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I
- Removal of this stain in-gel digestion and ligation techniques is not needed
- SYBR Green I is a greener alternative product to ethidium bromide for staining
Storage and Stability
Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days.
Legal Information
SYBR is a registered trademark of Life Technologies

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Sigma-Aldrich S9430 SYBR® Green I nucleic acid gel stain 10,000 x in DMSO
Sigma-Aldrich S9430 SYBR® Green I nucleic acid gel stain 10,000 x in DMSO 1 mL Sigma-Aldrich S9430 SYBR® Green I nucleic acid gel stain 10,000 x in DMSO 1 mL LB.SA.S9430-1ML
Sigma-Aldrich S9430 SYBR® Green I nucleic acid gel stain 10,000 x in DMSO 1 mL

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