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ZYMO RESEARCH D5325 5-mC DNA ELISA Kit 1 x 96 Preps
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ZYMO RESEARCH D5325 5-mC DNA ELISA Kit 1 x 96 Preps

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LB.MB.ZYMD5325
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ZYMO RESEARCH D5325 5-mC DNA ELISA Kit 1 x 96 Preps  
For high-throughput detection of global 5-methylcytosine (5-mC) in DNA
The streamlined workflow can be completed in less than 3 hours.

ZYMO RESEARCH D5325 5-mC DNA ELISA Kit 1 x 96 Preps  
The 5-mC DNA ELISA Kit allows researchers to accurately quantitate 5-mC in any DNA sample in less than 3 hours. The kit features an Anti-5-Methylcytosine monoclonal antibody that is both sensitive and specific for 5-mC. The assay is compatible with a wide range of input DNA from vertebrate, plant, and microbial sources as well as methylated PCR amplicons and fragmented DNA. Percent 5-mC in a DNA sample can be accurately quantified from a standard curve generated with specially designed controls included with the kit. Also, the fast, streamlined workflow is ideal for high-throughput analyses.
Detection > 0.5% 5-mC per 100 ng DNA
Equipment Incubator and ELISA plate reader. A multi-channel pipettor is recommended. An automated plate washer may be used for blocking and wash steps.
Input This protocol is optimized for 100 ng input DNA/well. Compatible with DNA in the range of 10-200 ng/well.
Sample Source Purified genomic DNA, plasmid DNA, PCR amplification products, or DNA fragments in water, Tris-EDTA, or similar.
 ZYMO RESEARCH D5325 5-mC DNA ELISA Kit 1 x 96 Preps ZYMO RESEARCH D5325 5-mC DNA ELISA Kit 1 x 96 Preps
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ZYMO RESEARCH D5325 5-mC DNA ELISA Kit 1 x 96 Preps

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